FTIR, BACTERIAL, AND ANTIBIOTICS RESISTANCE PROFILE OF LEAF EXTRACTS AGAINST PATHOGENIC CLINICAL ISOLATES OF Staphylococcus aureus FROM THE HUMAN WOUND

Abstract

In many underdeveloped nations, plant material is still used as therapeutic medicines in primary
health care. Thus, the focus of this research is on the discovery of medicinal plants as antibacterial
agents that can be used to broaden the range of antibiotics available. Plant extracts of (Ocimum
gratissum, Moringa oleifera, Momordica charantia, Azadirachta indica, and Citrus auratifolia)
were screened for antibacterial activities against Staphylococcusaureus (SA) isolated from human
wounds in two different years (2022 and 2023) at different seasonal weather. The leaves were
extracted by hand-crushing while 0.1ml was used against SA. The zone of inhibition (ZOI) was
recorded after 24 hours of incubation in millimeters (mm). The antibacterial effects of the leaf
extracts increase during the rainy seasons (13-24mm) than in the dry season (10-16mm). All the leaf
extracts have the highest ZOI (18mm-24mm) than the commercially sold antibiotics at the same
concentrations. Antibiotics sensitivity test result shows 100%, 97%, and 93% resistant to SA. In the
examined extracts, FTIR spectroscopy analyses revealed the presence of compounds that are
responsible for the activities found by identifying their groups

Keywords: Resistance profile, Leaf extracts, Clinical isolates, sensitivity, and Staphylococcusaureus.